Worksheet IV: Muscle System

1. The study of muscles is called:     _____________________________
2. Name 4 characteristics of muscle tissue.
         _____________________________
         _____________________________
         _____________________________
         _____________________________
3. A ___________________ stimulus is strong enough to initiate a response while a _________________ stimulus is not strong enough to initiate a response.
4. List 3 functions of muscles.
         _____________________________
         _____________________________
         _____________________________
5. Any change in one's internal or external environments is a(n):____________________________.
6. ATP  +  Eact -----------------> _______     +   __________    +  ____________.
7. Identify the muscle type based on the descriptions given below.
     a. Multinucleate.      _____________________________
     b. Cell is spindle-shaped.     _____________________________
     c.  Striated and uninucleate.     _____________________________
     d. Usuually attached to bone.     _____________________________
     e.  Unstriated.       _____________________________
     f. Found in the heart.      _____________________________
     g. Anastomosing fibers.     _____________________________
     h. Peripherally located nuclei.     _____________________________
     i. Centrally located nucleus.     _____________________________
     j.  Muscle fibers elongate in shape; voluntary.  _____________________________
     k. Intercalated discs present.     _____________________________
     l. Found in the walls of visceral organs.   _____________________________
8. Rank the following structures from largest to smallest: muscle fiber, myofibril, myofilament, muscle belly, fascicle, and sarcomere.
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9. Identify the parts of muscle macrostructure based on the descriptions given below.
     a. Large middle region of the muscle.   _____________________________
     b. Connective tissue that wraps around a fascicle.  _____________________________
     c.  Discrete bundle of muscle cells separated by connective tissue.      _____________________________
     d. Muscle fiber.      _____________________________
     e. Connective tissue wrapping around an individual muscle cell.       _____________________________
     f. Connective tissue wrapping around whole muscle. _____________________________
     g. Protective and provide entrance and exits for blood vessels and nerve fibers.     _____________________________
     h. Cord of connective tissue that attaches muscle to periosteum of bone.      _____________________________
     i.  A broad, flat tendon.      _____________________________
     j. Fixed point of attachment for muscle.   _____________________________
     k. Movable point of attachment for muscle.  _____________________________
10. Inflammation of tendon sheaths and synovial membranes of joints is called:_________________.
11. Identify the parts of muscle microstructure based on the descriptions given below.
     a. Muscle fiber.      _____________________________
     b. Cytoplasm of a muscle cell.     _____________________________
     c. Smooth endoplasmic reticulum of a muscle cell.  _____________________________
     d.  Plasma membrane of muscle cell.   _____________________________
     e. Main source of ATPs for muscle metabolism.  _____________________________
     f. Stores Ca++.      _____________________________
     g. Transverse indentations of the sarcolemma.  _____________________________
     h. Enlarged sac-like end of the SR.   _____________________________
     i. Elongate contractile structures in a muscle cell.  _____________________________
     j. Functional unit of muscle contraction.   _____________________________
     k. Conducts action potential towards interior of cell. _____________________________
12. Identify the parts of a myofibril based on the descriptions given below.
     a. Area between 2 adjacent Z lines.   _____________________________
     b. Thick myofilament.      _____________________________
     c. Thin myofilament.      _____________________________
     d. Area of sarcomere that represents length of thick myofilaments.       _____________________________
     e. Area divided by a Z line that includes thin myofilaments. ___________________________
     f. Disc-like protein sheet used for attachment of actin proteins.________________________
     g. Light area in the middle of an A band.   _____________________________
     h. Area of A band where actin is absent.   _____________________________
13. A myosin myofilament is composed of ~ 200 molecules of the protein __a__.  Each __a__ protein is shaped like a two-headed golf club.  The shaft is referred to as the __b__ while the heads are referred to as __c__ or __d__.  These 200 molecules of __a__ are bundled together with the __b__ pointing to the __e__ (ends/middle)  and the __c__ facing __f__ (in/out).  Because of this arrangement the middle of the myosin myofilament without __d__ is known as the __g__.
 a. ___________________________   e. ___________________________
 b. ___________________________   f. ____________________________
 c. ___________________________   g. ___________________________
 d. ___________________________
14. An actin myofilament is composed of 3 proteins.  The main protein is called __a__.  __a__, also known as __b__, is polymerized from __c__ .  Each __c__ is globular in shape and has an __d__ site that bonds with the myosin head.  __e__ (1, 2, 3, etc.) strands of __b__ coil around each other.  A second rod-shaped protein called __f__ spirals around the __a__ helix.  At evenly spaced increments along the length of the actin myofilament is a third protein called __g__.   __g__ is composed of 3 polypeptides:  the __h__ unit binds to the actin; the __i__ unit binds to the __f__, and the third unit called __j__ binds __k__ ions.
 a. ___________________________   f. ___________________________
 b. ___________________________   g. ____________________________
 c. ___________________________   h. ___________________________
 d. ___________________________   i. ____________________________
 e. ___________________________   j. ____________________________
         k. ____________________________
15. The cross-bridges of the myosin myofilaments act like which enzyme? _____________________
16. List 3 observations available to researchers that contributed to the formulation of the sliding filament theory of muscle contraction.                   ___________________________________
        ___________________________________
        ___________________________________

17. List, in the correct sequence, the 4 main events that cause actin and myosin to slide past each.
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18.    Fill in the blanks regarding the steps of the sliding filament theory.
 A resting muscle cell has a low concentration of __a__ ions in its __b__.  __a__ ions are in high concentration in the __c__.  When __a__ ions are in low concentration, myosin-binding sites on the G actins are covered by __d__ molecules.  Stimulation from a nerve impulse causes __a__ ions to be released from the __c__.  __a__ ions bind to __e__ and cause a conformational change that physically removes __d__ from blocking active sites (=myosin-binding sites) on G actins.  The myosin __f__ are attracted to the exposed (active) sites. This event is called __g__.  The myosin __f__ changes from a  __h__ (high/low) energy state to a __i__ (high/low) E state. This event is called the __j__. The __j__ pulls on the thin filament and slides it towards the center of sarcomere. When the __j__ reaches the __k__ (high/low) energy state, __l__  generated during the previous  contraction cycle, are released from the myosin __f__.  A new __m__ binds to the myosin __f__ and this releases the __f__ from the actin binding sites.  This event is called __n__.   The myosin __f__ enzymatically breaks down the __m__ molecule and the released energy is used to change the myosin __f__ back to the __o__ (high/low) state.  This event is called __p__. The events __g__, __j__, __n__, and __p__ are repeated until maximal shortening is acheived.  The cycle is terminated when nervous stimulation stops and a __q__ removes Ca++ from the __b__.
 a. ___________________________   j. ___________________________
 b. ___________________________   k. ____________________________
 c. ___________________________   l. ___________________________
 d. ___________________________   m. ___________________________
 e. ___________________________   n. ____________________________
 f. ___________________________   o. ___________________________
 g. ___________________________   p. ____________________________
 h. ___________________________   q. ___________________________
 i. ___________________________
19. A single power stroke gives a ____% reduction in muscle cell length, but a muscle shortens to between __________ %.
20. Only about _________ (1/8,1/4, 1/2, etc.) the myosin heads are bonding at any one instant.
21. __a__ is the stiffening of muscles several hours after death. Dead cells cannot keep __b__out of the sacromere, the availability of these ions results in __c__. With depletion of __d__ stores and the inability to synthesize to more __d__ the __e__ cannot detach.  Bacterial breakdown of __f__ causes the body to become limp.
 a. ___________________________   d. ___________________________
 b. ___________________________   e. ____________________________
 c. ___________________________   f. ___________________________
22. The series of events by which electrical excitation of the sarcolemma leads to contraction is called: _____________________________.
23. The junction between a nerve cell and a muscle cell is called a(n): ____________________ junction.
24.  Identify the parts of the myoneural junction based on the descriptions given below.
     a. The enlarged terminal end of a nerve cell.  _____________________________
     b. Space between end of nerve cell and muscle cell. _____________________________
     c. Region of sarcolemma at myoneural junction.  _____________________________
     d. Structures within enlarged terminal end of nerve cell that house neurotransmitter.     _____________________________
25. Calsequestrin is a protein that:   _________________________________________
26.  ________________________ is the neurotransmitter released at myoneural junctions.
27.  _________________, a S. American tree resin is a competitive inhibitor with ACh.
28.  ___________________ is an autoimmune disease that results in the production of antibodies that block ACh receptors.
29. ___________________ is a protein found in the sarcoplasm that binds Ca++ ions.

30. A nerve impulse arrives at the end of the axon in a region called the __a__. The __a__ houses __b__ that contain the neurotransmitter __c__.  This nerve impulse causes an influx of __d__ ions that trigger a series of events resulting in the __e__ of __c__ into the space between cells called the __f__.  __c__ diffuses across the __f__ and binds to receptors on the __g__.  This may result in __h__ of the sarcolemma.  This wave of __h__ travels to the interior of the muscle by the __i__ system and causes the __j__ of the sarcoplasmic reticulum to release __k__ ions into the __l__.  The __c__ on the __g__ is destroyed by the enzyme __m__.  __k__  ions bind to troponin C and initiate the evnts of muscle contraction.
 a. ___________________________   h. ___________________________
 b. ___________________________   i. ____________________________
 c. ___________________________   j. ___________________________
 d. ___________________________   k. ___________________________
 e. ___________________________   l. ____________________________
 f. ___________________________   m. ___________________________
 g. ___________________________
31. A motor neuron and all the muscle fibers it innervates is called a(n):_______________________.
There are as few as _____ (1, 2, 3, etc) of these units in eye muscles and as many as several  ____ (dozen, 100's, 1000's etc) in thigh muscles.
32. Muscle fibers in a single motor unit  _______________ (are not/are) clustered together.
Therefore, stimulation of a single unit gives a _______ (strong/weak) contraction throughout the entire muscle.
33. Answer the following questions regarding a muscle twitch or graded responses.
     a. Graphical recording of muscle activity .   ____________________________
     b. Two stimuli of identical strengths given in rapid succession, the second before the relaxation phase is
     completed; second wave rides on first.   ____________________________
     c. Response of a muscle to a single threshold stimulus. ____________________________
     d. Shortening of muscle fibers to peak tension-lasts 10-100 ms. ______________________
     e.  Increasing voltage given brings in more motor units.  ____________________________
     f.  Time following stimulation where excitation-contraction coupling begins; lasts a few ms.    ____________________________
     g.  Some motor units are contract while others are relax producing a smooth, even contraction.   ____________________________
     h. Many stimuli of the same strength given in rapid sucession before relaxation period is complete.   ____________________________
     i. Many stimuli of the same strength given in rapid sucession at end of contraction phase, just before relaxation period
     starts.        ____________________________
     j.  Lengthening of muscle fibers to original state; tension returns to zero-lasts over 100 ms.   ____________________________
     k. Multiple stimuli of the same strength given in rapid sucession, immediately after relaxation period is
     complete; Produces staircase effect.    ____________________________

34. An involuntary, painful, prolonged contraction is called a(n): ____________________________.
35. Give 2 causes for treppe and wave summation.
         ____________________________
         ____________________________
36.Force exerted by muscle on some object is termed:  ____________________________
37. Resisting force or weight is termed  the:   ____________________________
38. Movement is accomplished when the ________________ exceeds the __________________.
39. If the load is greater than the tension the contraction is: ____________________________.
40. If the tension exceeds the load and the load moves the contraction is: ____________________.

41. The greatest tension is produced when the maximum number of __a__ link actin and myosin myofilaments.  Stretching beyond 175% of muscle resting length results in __b__  (no, some, maximal) tension while lengths less than 50% of resting length jam the myosin myofilaments into the __c__ and __d__ (reduces/increases) muscle tension.
 a. ___________________________   c. __________________________
 b. ___________________________   d. __________________________
42. During strenuous muscle activity muscle cells use 3 energy molecules.  Name these in the order they are used.
         _____________________________
         _____________________________
         _____________________________
43.  Creatine phosphate  +  _____  ------------(Ez: _______________)-----> _____________ +     ATP
44.  Normal muscle activity uses _________________  to generate ATPs.
45. Name the 3 major pathways in cellular respiration.
         _____________________________
         _____________________________
         _____________________________
46.  During strenuous activity, metabolism shifts from fatty acids to: ________________________.
47.  The endproduct of glycolysis is:     ____________________________.
48.  Two energy molecules produced by glycolysis are:
         ____________________________
         ____________________________
49. Glycolysis is an ____________________  (aerobic/anaerobic) pathway.
50. If oxygen is absent pyruvate is converted to:  ____________________________.
51. If oxygen is present the pyruvate enters the:  ____________________________.
52.  Name 3 energy molecules produced from the Kreb's Cycle.
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         ____________________________
         ____________________________
53. Two waste products formed by the Kreb's Cycle are: _______________ and ______________.
54.  The NADH2's and FADH2's produced by glycolysis and the Kreb's Cycle provide ______________ and _______________ for the electron transport system.
55. The ETS generates ATPs by the process called:  ____________________________.
56. Nearly _____ (2, 3, 4 etc.) times more ATPs are produced during aerobic as opposed to anaerobic respirations.  However, ATP are made approximately ______ (2, 3, 4 etc.) times faster by __________ respiration.
57. The physiological inability of a muscle to contract is:  ___________________________.
58. Name 3 factors that contribute to muscle fatigue.
         ___________________________
         ___________________________
         ___________________________
59. ________________________ is the extra amount of oxygen that must be taken in by the body to provide for restoring lowered reserves of ATP, CP, glycogen and for removal of lactic acid.
60. Identify the type of skeletal muscle fiber based on the descriptions given below.
     a.  Very high myoglobin content; Splits ATP rapidly. _____________________________
     b.  High myoglobin, Splits ATP slowly.   _____________________________
     c.   Low myoglobin and few capillaries.   _____________________________
     d.   Splits ATP rapidly; large glycogen reserves.  _____________________________
     e.  Found in eye and hand muscles; Many capillaries. _____________________________
     f.  Few mitochondria, fatigue easily.   _____________________________
     g.  Color pale or white; Contraction velocity fast.  _____________________________
     h.  Many mitochondria and capillaries; contraction velocity slow.        _____________________________
     i. Postural muscles; leg muscles.     _____________________________
61. Muscles increase in size by: (synthesis of new myofibril units/division of muscle cells to form more cells/ or a combination of the first 2 methods).
______________________________________________
62. List 4 benefits of exercise affecting muscle tissue.
         _____________________________
         _____________________________
         _____________________________
         _____________________________
63. Muscles that are not used will decrease in size; this condition is called _____________________.
64. List 10 differences between smooth and skeletal muscles.
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65. Z-line counterparts in smooth muscle are dark-staining structures called: __________________.
66. Smooth muscle cells lack refined myoneural jcts; these areas are called: _____________________.
67.  Smooth muscle cells are often arranged in sheets. The alternate contractions of these sheets produces a movement called:      ____________________________.
68.  List 3 ways in which skeletal and smooth muscles are alike.
         _____________________________
         _____________________________
         _____________________________
69.  The main source of Ca++ for smooth muscle contraction is from the: _____________________.
70.  Smooth _______ (can/cannot) generate similar tensions to skeletal muscle.
71. Smooth uses about _______ % the energy as skeletal muscle.
72. Name 2 neurotransmitters released at smooth muscle bulbous varicosities.
         ____________________________
         ____________________________
73.  A smooth muscle contraction can be initiated by 3 mechanisms. Name these mechanisms.
         ____________________________
         ____________________________
         ____________________________
74.  Smooth muscle will contract vigorously in response to a stimulus, but will then relax at a longer resting length.  This phenomenon is referred to as the: ____________________________.
75. Skeletal muscle functions best 30% below to 30% above its resting length. Smooth muscle has a __________________ (wider/narrower) range.
76. Smooth muscle cells divide and increase in number, a characteristic called:__________________.
The hormone ____________________ will cause this to occur.
77. Smooth muscle cells can make new cells, contractile proteins, and elastin and collagen for surrounding connective tissues, a feature called:  _____________________________.
78. What kind of cell normally manufactures elastic and collagen fibers for connective tissues?
         _____________________________

79. Identify the kind of smooth muscle based on the descriptions given below.
     a. Most common.      _____________________________
     b. Fibers structurally independent.   _____________________________
     c.  Large airways and large arteries; arrector pili, internal eye muscles.       _____________________________
     d.  Cells arranged in sheets-united by gap jcts.  _____________________________
     e.  Contracts as unit and with rhythm.   _____________________________
     f. Also called visceral muscle.     _____________________________

80. Explain the rationale (at the molecular level) behind stretching and warming up before an athletic event. _______________________________________________________________________
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81. What is the power stroke and how does it cause a muscle to shorten? ____________________
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82. What is the difference between a graded response and a muscle twitch? Use two graphs to explain your answer. ____________________________________________________________
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83. What is excitation-contraction coupling (i.e. events at the myoneural junction)? Briefly summarize the steps involved._____________________________________________________________
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84. Organophosphate pesticides block the action of the enzyme AChE.  What symptoms would a person have if they are poisoned by these pesticides and why?
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85. Why does skeletal muscle have striations and smooth muscle does not? __________________
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